Quantification and FTIR-based chemical characterization of condensed tannins extracted from the bark of tropical tree species

Luan Cavalcanti da Silva; Paula Evanyn Pessoa do Nascimento; Kayo Lucas Batista de Paiva; Denys Santos de Souza; Alexandre Santos Pimenta; João Gilberto Meza Ucella-Filho; Tatiane Kelly Barbosa de Azevedo

doi:10.53661/1806-9088202549263864

Authors

Luan Cavalcanti da Silva Universidade Federal do Rio Grande do Norte, Mestrado em Ciências Florestais, Macaíba, Rio Grande do Norte, Brasil https://orcid.org/0009-0007-4183-1838
Paula Evanyn Pessoa do Nascimento Universidade Federal do Rio Grande do Norte, Programa de Pós-Graduação em Ciências Florestais, Macaíba, Rio Grande do Norte, Brasil https://orcid.org/0009-0007-9455-1493
Kayo Lucas Batista de Paiva Universidade Federal do Rio Grande do Norte, Programa de Pós-Graduação em Ciências Florestais, Macaíba, Rio Grande do Norte, Brasil https://orcid.org/0009-0007-4102-5844
Denys Santos de Souza Universidade Federal do Rio Grande do Norte, Graduado em Engenharia Florestal, Macaíba, Rio Grande do Norte, Brasil https://orcid.org/0009-0002-8043-7167
Alexandre Santos Pimenta Universidade Federal do Rio Grande do Norte, Programa de Pós-Graduação em Ciências Florestais, Macaíba, Rio Grande do Norte, Brasil https://orcid.org/0000-0002-2134-2080
João Gilberto Meza Ucella-Filho Universidade Federal de Viçosa, Departamento de Engenharia Florestal, Viçosa, Minas Gerais, Brasil https://orcid.org/0000-0001-9797-3332
Tatiane Kelly Barbosa de Azevedo Universidade Federal do Rio Grande do Norte, Programa de Pós-Graduação em Ciências Florestais, Macaíba, Rio Grande do Norte, Brasil https://orcid.org/0000-0001-6392-3548

DOI:

https://doi.org/10.53661/1806-9088202549263864

Keywords:

Phenolic compounds, Functional groups, Bark valorization, Biorefinery

Abstract

The sustainable exploitation of forest species contributes to biodiversity conservation while generating income, with tannins being one of the valuable products obtained. These biodegradable phenolic compounds have multiple industrial applications, including leather tanning, wastewater treatment, adhesive production, and use in pharmaceutical and cosmetic products. The quantification and chemical characterization of condensed tannins are essential to identify species with commercial potential and determine their possible applications. This study aimed to quantify and chemically characterize the condensed tannins extracted from the bark of four tropical tree species. Bark samples from Acacia mangium, Copaifera arenicola, Hancornia speciosa, and Terminalia catappa were collected, dried, and ground to reduce particle size. The ground material was subjected to hot water extraction under boiling conditions using distilled water. Subsequently, total solids content (TSC), Stiasny index (SI), and condensed tannin content (CTC) were determined. The extracted tannins were dried to a solid form and analyzed by Fourier-transform infrared (FTIR) spectroscopy to identify the functional groups present. The SI of the analyzed species ranged from 95.34% to 55.09%, with A. mangium showing the highest SI and H. speciosa the lowest. However, despite the higher SI of A. mangium, it exhibited the lowest CTC (6.30%), while C. arenicola showed the highest CTC (14.86%). The hydroxyl (OH) group was identified in all samples by FTIR, confirming the presence of phenolic compounds such as tannins. The studied species demonstrated potential for tannin production and extraction from bark, with possible applications across various sectors. Furthermore, considering that bark is often regarded as waste, its valorization underscores the importance of further research aimed at optimizing extraction processes and promoting the sustainable use of these natural resources, with significant ecological and economic implications.

Keywords: Phenolic compounds; Functional groups; Bark valorization; Biorefinery